Page 81 - Feed Lab

Estimation of Phosphorus Suguna Management System Ver 1.0 / SOP / FQL / P2 - 10 Page 2 of 3

up to the mark with double distilled water.

FStandard Phosphorus solution: (80 micro grams
per ml) Take 0.0352g of potassium di hydrogen
ortho phosphate AR grade, dissolve in distilled
water and make up to 100 ml. Now this solution
contains 0.008g of phosphorus is present in 100
ml of standard phosphorus solution other wise 80
micro gram of phosphorus is present in one ml of
Picture-7 Picture-8
standard phosphorus solution.

PROCEDURE

Test solution for DCP/MCP
FDissolve accurately 1g of DCP / MCP sample, add
1:3 hydrochloric acid till effervescence ceases and
add 5 drops of 1:3 nitric acid. Give slight boiling to
the solution and cool. (Picture - 13)

Picture-9 Picture-10
FThen filter through filter paper with distilled water
and wash the filter paper with distilled water and
collect the filtrate in a 250 ml volumetric flask and
make up volume. (Picture - 14)

Test solution for finished feed:
FWeigh accurately 2g of sample in a silica crucible
and keep it in a muffle furnace for 1½ hour at
o
o
600 C+/-20 C. Remove the crucible from the
furnace and cool. (Picture - 15)
Picture-11 Picture-12
FTransfers the ashes to a 250 ml beaker add 1:3
hydrochloric acid till effervescence ceases and add
REAGENTS
F 15% Sodium meta bi sulphate or sodium bi 5 drops of 1:3 nitric acid. Give slight boiling to the
sulphate (w/v), AR grade 20% Sodium sulphite solution and cool. Then filter through filter paper
(w/v), AR grade with distilled water and wash the filter paper with
distilled water and collect the filtrate in a 250 ml
F Preparation of ANSA: Weigh 25 mg of ANSA in a
volumetric flask and make up volume. (Picture - 16)
test tube, add 0.25 ml of 20% sodium sulphite and
9.75 ml of sodium Meta bi sulphate, shake well and Test solution of DCP, MCP, Dry fish and Meat &
filter. Bone meal:

F Ammonium Molybdate solution: Take 2.55g of FPipette 25 ml of test solution in 100 ml volumetric
Ammonium Molybdate, AR grade in a 100 ml of flask and make up to volume. (Picture - 17)
volumetric flask and dissolve in 8.23 ml of
concentrated sulphuric acid, AR grade and make

Prepared by : Approved by :
69 Director - Technical