Page 78 - Feed Lab

Estimation of Calcium Suguna Management System Ver 1.0 / SOP / FQL / P2 - 09 Page 2 of 2

FTransfer a 25 ml aliquot to a 250 ml beaker and add
75ml of distilled water with 2drops of methyl red
indicator solution. Add 50% ammonium hydroxide
solution drop wise till brownish orange colour
obtained. Add 1 or 2 drops of concentrated
hydrochloric acid so that the colour of the solution
is pink. Add 45ml of distilled water and bring it to
boil. (Picture - 10)

FAfter boiling, add 10ml hot saturated ammonium
Picture - 6 Picture - 7
oxalate slowly with constant stirring. The stirring
time should be one minute. If the red colour of the
REAGENTS
solution changes to orange or yellow, add
FConcentrated Hydrochloric acid. concentrated hydrochloric acid drop wise until the
colour again changes to pink. (Picture - 11)
FMethyl red indicator
FLeave for minimum 4 hours and allow the
F50% Ammonium Hydroxide solution (v/v).
precipitate to settle. Filter the supernatant liquid
F2% Ammonium Hydroxide solution (v/v). through ash less filter paper (Whatman No.1/615A)
and wash the precipitate thoroughly with 250ml of
FAmmonium oxalate solution-saturated.
2% ammonium hydroxide solution. (Picture - 12)
FConcentrated sulphuric acid, AR grade
FPlace the paper with the precipitate in mixture of
F0.1N Potassium permanganate (3.2 g / 1litre) AR 125 ml of distilled water and 5 ml of concentrated
o
o
grade. sulphuric acid in a beaker. Heat 70 C to 90 C and
titrate with the standard potassium permanganate
solution until the first slight pink colour is obtained.
Standardization of std. Potassium permanganate
(Picture - 13)
Take 125ml of distilled water with 5ml of concentrated
sulphuric acid in a beaker and add 10ml of 0.1N oxalic
o
o
acid. Heat above solution to 70 C - 90 C and Titrate
against potassium permanganate.

PROCEDURE
FWeigh approximately 1g for mineral supplement
and 2g for feed and feed stuffs in a silica crucible.
Ignite the sample on a burner till smoke cease off.
o
o
Ignite at 600 C +/- 20 C in muffle furnace for 90
minutes to get carbon free ash. Remove the Picture - 8 Picture - 9
crucible from the furnace carefully and cool.
(Picture - 8)

FTransfer the ash to the 250 ml beaker and add 20
ml of 1:3 hydrochloric acid till effervescence
ceases and add 5 drops of 1:3 nitric acid. Give
slight boiling to the solution and cool. Transfer
through filter paper (Whatman No.1/615A) to 250
ml volumetric flask. Cool, dilute to the mark and mix
thoroughly. The solution is also to be used for the
Picture - 10 Picture - 11
estimation of Phosphorus. (Picture - 9)

Prepared by : Approved by :
66 Director - Technical