Page 82 - Feed Lab

Estimation of Phosphorus Suguna Management System Ver 1.0 / SOP / FQL / P2 - 10 Page 3 of 3

Preparation of Standard and test solution for absorption in calorimeter: Take a set of test tube for the
following serial solution.

Blank S1 S2 S3 S4 T1 T2 T3 T4
Std. P - 0.1 0.2 0.3 0.4 - - - -
Test sol. 1 - - - - - 0.1 0.1 0.1 0.1

Test sol. 2 - - - - - 0.2 0.2 0.2 0.2
Water 1 3.0 2.9 2.8 2.7 2.6 2.9 2.9 2.9 2.9
Water 2 3.0 2.9 2.8 2.7 2.6 2.8 2.8 2.8 2.8
Amm.Molyb 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0

ANSA 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4

Note : Test sol.-1 and water-1 for DCP / MCP, test sol.-
2 and water-2: for feed, dry fish, MBM etc.

FMix the contents in the test tube using cyclo mixer
and let the test tube stand for 20 minutes in dark
room. (Picture - 18)

FMeasure Optical Density (OD) of standard and test
solution against blank using a Colorimeter at 660
nm.
Picture - 15 Picture - 16

Picture - 13 Picture - 14
Picture - 18
Picture - 17
CALCULATION

O.D of sample x concentration of P x 250 x 100
Total Phosphorous % = -----------------------------------------------------------------------------------------
O.D of standard x aliquot x 1000 x 1000 x sample weight, g

REFERENCE
1. Bureau of Indian Standard IS 7874 1975
2. Association of Official Analytical Chemist 2000.

Prepared by : Approved by :
70 Director - Technical