Page 14 - Abstract Booklet-TRiO 2020 Virtual Explorations

INFORMATIVE ABSTRACT

Group Members

Kyandre Samuel
Walker Charlelemange

Using Skeletal Muscle Fibers To Generate iPSCs And Differentiating Them
Into Neurons

INTRODUCTION:
Advances in Induced Pluripotent Stem Cells (iPSCs) have been discovered as an alternate to embryonic stem

cell extraction and has opened up a gateway to many research topics, such as this study in taking fully

differentiated skeletal muscle cells and turning them into iPSCs and making neurons from them. This study
hypothesized that there would be a large success rate of cell change to measure if there were large numbers of

multinucleated muscle cells compared to the amount of uninucleate neuron cells to show.

METHODS AND PROCEDURES:

Starting with lab made skeletal muscle tissue using transcription factors in the MyoD family, an adenovirus to
insert Oct4, Sox2, Klf4, and c-Myc transcription factors (Tfs), reverted the muscle cell into an iPSCs. Once that

was achieved, neural transcription factors Ztf-11, Mytl-1, MuvB, and Sin3 along with other TFs were added to

form neurons.

RESULTS:

Results showed a success rate for large and pliable cell change supporting the hypothesis that there would be a

large success rate of cell changes to measure, if there would be any multinucleated muscle cells compared to the
amount of uninucleate neuron cells to show.