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Large/Coarse speckled pattern

(AC-5)

Hep-2 cells, generally show a coarse speckled, sometimes

medium to fine speckled fluorescence, which is spread over

the entire cell nucleus, leaving the nucleoli free. In mitotic

cells the condensed chromosomes are dark, while the

periphery shows an almost homogeneous, smooth

fluorescence.

Primate liver, also show a speckled fluorescence. the

nucleoli do not react. the antibodies react with primate liver

to the same extent as with HEp-2 cells.