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Avian Reovirus | 181

Figure 6.1 Phylogenetic tree showing the relationships within family Reoviridae. Nucleotide sequences of RdRp gene of selected members
in the different genera of family Reoviridae were used to generate a phylogenetic tree. The sequences of member viruses used for comparison
are identified by the genus name/GenBank accession number [Piscine reovirus/GU994015, KC795567, KC715681, KC776258, LC145609,
KU974955; Orthoreovirus/JF342673, DQ534201, EU707935, M24734, NC_015878, KF306083.1, NC_029912; Aquareovirus/JN967630,
HQ231199, EF434978, NC_010585, AF260512, NC_005167; Mimoreovirus/NC_008172; Orbivirus/AF133431, U94887, JQ972851, L20446,
GU390658; Cardoreovirus/AY542965; Phytoreovirus/NC_003773, NC_012535; Seadornavirus/NC_004211, NC_004210; Fijivirus/D49693,
NC_003729, AF499925; Rotavirus/GQ414540, X16830; Idnoreovirus/DQ192235; Oryzavirus/HQ012655, NC_003771; Cypovirus/AY496445,
NC_010668, DQ192251; Dinovernavirus/NC_007667, KM978416; Mycoreovirus/AB102674, AY277888; Coltivirus/NC_004181, AF282476.
Scylla serrata reovirus (SsRV)/HM807276 (Chen et al., 2011a) is not yet assigned to a genus, and there is a new proposed genus Crabreovirus/
HQ414127 (Shen et al., 2015)]. Sequences were processed using ClustalX 2.0 (Larkin et al., 2007). The multiple sequence alignment was
manually verified and adjusted to reach high quality alignment. The phylogenetic tree was constructed by maximum likelihood using the
neighbour-joining method and Tamura-Nei genetic distances (Saitou and Nei, 1987). Bootstrapping was performed for 1000 times. Bootstrap
supports of topology are shown below the nodes, only for nodes between genera. The scale bar represents the number of substitutions per
site.