Page 249 - The Manga Guide to Biochemistry

Transferred Membrane
protein bands
When viewed directly
from the side...
Sugar chains
are attached.

Lectin

GlcNAc

When using the property j All proteins are stained with CBB.
of a lectin that reacts k Only proteins possessing sugar
to GlCNAc...
chains with GlCNAc attached are
Membrane stained because of WGA (arrows).
l These proteins are stained with
Bingo! concanavalin A, which is another
lectin.
the reagent that bonded to the
lectin glows. Only proteins that (Photos provided by: Mitsutaka
Ogawa, Nagahama Institute of
have sugar chains with GlCNAc Bio-Science and Technology)
attached will be detected.

Centrifugation

Like column chromatography, centrifugation is an experimental method that is performed
to separate organelles, proteins, and other biological molecules that have the same density
from a mixture of substances with differing densities. The sample solution is placed in a test
tube, and the test tube then spins around the centrifuge axis at a very high speed. To handle
small molecules, like proteins, ultracentrifugation may also be performed, which spins the
test tube at tens of thousands of revolutions per minute. DNA and other polymers can also
be isolated in this way.

Place the Micro test which
tube in the centrifuge... Revolves at a

high speed.

Mixture of various approximately Substance A
substances 40 mm Substance B

Small container such as Centrifuge Isolated samples
a micro test tube

Molecular Biology and the Biochemistry of Nucleic Acids 235