Page 264 - The Manga Guide to Biochemistry
P. 264
cholesterol ester, 101 DNA (deoxyribonucleic acid), 17, reaction measurement of
chromatography of columns, 202–203, 218–219 a-amylase activity
method, 237
232–233 DNA polymerase I, 212 DNA polymerase activity
citric acid cycle (TCA cycle), 67, DNA polymerase activity mea- method, 236
overview, 236
71–73, 115 surement method, 236
CoA (coenzyme A), 85 double bonds, 96 substrates and, 162–165
transferases
acetyl-CoA, 72, 85, 115–117, E
120–122 determining blood type,
EC Number (Enzyme Commission 169–171
acyl-CoA, 120–121 Number), 166
malonyl-CoA, 116–117 overview, 168
succinyl-CoA, 72 ecosystems using graphs to understand
column chromatography, biogeochemical cycle, 40–44
carbon cycle, 45–47 activation energy, 176
232–233 calculating Vmax and Km,
competitive inhibition, 193 electron transport chain, 52–55,
complementarity 67, 74–78 182–192
chemical reactions,
configurations, 211 electrophoresis, 233–234
concentration gradient force, 77 endoplasmic reticulum, 16, 17 175, 177
consumers, 48 endothelial cells, 113 maximum reaction
covalent bonds, 36 energy
cycles rate, 178
ATP, 82 Michaelis-Menten equation
biogeochemical, 40–44 creation of
carbon, 45–47 and Michaelis constant,
citric acid, 71–73 citric acid cycle, 71–73 180–181
cyclic structure, 61, 63, 141 electron transport chain, overview, 174
cytidine monophosphate, 208 essential fatty acids, 95
cytoplasm, 16 74–78 ether extraction, 204
cytosine (C), 206, 208 glucose decomposition by eukaryotes, 227
cytosol, 16, 30 exons, 219, 227
glycolysis, 68–70 expended energy, 106–110
D overview, 66–67 experiments
fat used as, 118–122 centrifugation, 235
deoxyadenosine ingested and expended, column chromatography,
monophosphate, 207 232–233
106–110 electrophoresis and western
deoxycytidine metabolism, 38 blots, 233–234
monophosphate, 208 Enzyme Commission Number (EC enzyme reaction measurement
a-amylase activity
deoxyguanosine Number), 166 measurement
monophosphate, 208 enzymes method, 237
DNA polymerase activity
deoxyribonucleic acid (DNA), 17, classifications of, 166–167 measurement
202–203, 218–219 DNA polymerase, 212–214 method, 236
fundamentals of, 37 overview, 236
deoxyribose, 207 hydrolases, 172–173 lectin blotting, 234–235
deoxythymidine inhibitors and overview, 232
external respiration, 37, 65
monophosphate, 208 allosteric enzymes,
deoxyuridine monophosphate, 208 196–199
detoxification, 21
D-form isomers, 84 overview, 193–195
diacylglycerol, 92 lipoprotein lipase, 112
dietary fiber, 142 proteins and
digestion, 7
amino acids, 154–161
overview, 150–151,
153–154
roles of, 151–152
250 Index
