Page 110 - Book of Abstracts

th
8 Biannual Conference on Chemistry - CHEM 08

Identification of Volatiles Components of Honey from Local

Market in Saudi Arabia Using HS-MSPE Followed by GC-
MS

Manahel A. Khouja, Saleh O. Bahaffi
Chemistry Department, Faculty of Science, King Abdulaziz University,
Jeddah-Saudi Arabia/ Faten M. Ali Zainy, Chemistry Department,
Faculty of Science, University of Jeddah, P.O. Box 80327, Jeddah 21589,
Saudi Arabia
Email: manahel.khouja@hotmail.com

ABSTRACT
Honey has been proven to be excellent healing for many diseases. Their
properties are dependent mainly on their volatile fractions. Saudi markets
cover many types of honey; some are local and others imported. On this
study,volatile compounds on ten kinds of honey products with different
prices, sources, and botanic origin were analyzed. Using Head Space Solid
Phase Microextraction (HS-SPME), followed by Gas Chromatography-Mass
Spectrometry (GC-MS) for analysis. The sample composition is (1:1 w/v
honey to water ratio), 2g sample size. To obtain complimentary data for an
overall characterization of honey aroma, three different SPME fiber coatings:
100µm Polydimethylsiloxane (PDMS), 50/30µm Divinylbenzene/Carboxene
onpolydimethylsiloxane (DVB/CAR/PDMS) and 60µm Polyethylene glycol
(PEG) were employed (at temperature in range of 40-60 ◦C during an
extraction time of 40 min). These three fibers are evaluated and compared in
terms of a maximum signal obtained for total volatile compounds. The fiber
coatings DVB/CAR/PDMS showed the best Outcomes. By using this fiber
coating, up to 70 compounds belonging to different classes have been
separated and identified. These compounds including Hydrocarbons,
Alcohols, Organic acids, Aldehydes, Ketones, Phenols, Terpenoids, and
Derivatives of Furan. The main compounds of honey after the extraction
(DVB/CAR/PDMS) were: Furfural, Benzeneacetaldehyde, Terpinen-4-ol and
Lilac alcohol B. The proposed method is reliable and accurate for different
kinds of honey of various botanical origins.

BOOK OF ABSTRACTS CHEM 08 (2020) Page 109