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Carleton microbiologist Myron Smith is helping Ottawa’s Buchipop gear up for growth.


                                     The kombucha project

                         Understanding the fermented tea drink’s microbial mix

                                       is the key to a consistent product

          ts fiercest advocates claim that drinking kombucha can   the local, the drink’s
        Ihelp address a dizzying array of health problems, from dia-  regional specificity
        betes to a weak libido, but Ottawa’s Buchipop isn’t brewing   is a marketing asset,
        the fermented, fizzy tea-based beverage as a folk remedy —   but a challenge to
        they want to bring it to the soda-swilling masses.     growth. To expand,
          “Our focus isn’t really on the health side,” says Patricia   Buchipop needs to
        Larkin, who started the company in early 2016 after five   produce consistent
        years as a chef at one of the city’s top bistros. “That’s part   kombucha on a large
        of who drinks kombucha, but we want soda drinkers to love   scale, and a dynamic
        it too. I see us as a lifestyle brand. An alternative to soda,   production process
        with health benefits.”                                  with unknown micro-
          Kombucha traces its lineage back two millennia to ancient   bial components presents a hurdle to consistency.
        Manchuria, but despite this pedigree, little is known about ex-  Smith’s lab in the Nesbitt Biology Building is sequencing
        actly what it is. That’s because of kombucha’s microbial com-  the DNA in Buchipop’s symbiotic community of bacteria and
        plexity. Drinks such as wine and beer ferment a single yeast,   yeast using a high throughput sequencer. By examining the
        but kombucha relies on a symbiotic community of bacteria   metagenome — the genome of the entire community — the
        and yeast in a complex fermentation process that produces   lab can identify microbial DNA to determine the proportion
        an alcohol content of less than one perfect, similar to many   of a particular species in the mix, and identify exactly which
        other foods and beverages. To complicate matters, it uses an   components are present at each stage of fermentation. “You
        open system that allows external microbes to become part of   can imagine a pie chart that says 10 percent of the cells are
        that community, contributing unique local flavours.     from this bacterium, and 20 percent from this yeast,” Smith
          “You don’t try to keep it clean of environmentally intro-  says. “Then a week later, you can see how that community
        duced microbes,” says Carleton microbiologist Myron Smith,   has changed.”
        a genetics expert whose lab is working with Buchipop on a   Understanding what is happening as kombucha ferments
        research project. “Different regions have different microbes.   could enable Buchipop to better manage flavours in the end
        So there won’t be the same species, or the same strain, but   product, and achieve the consistency the growing company
        there will be something that lives well in a region that does   needs to expand beyond the roughly 40 local restaurants
        the same job.”                                         currently selling its products.
          With global sales forecast to climb 25 percent every year   “I’m very proud to be an Ottawa company,” says Larkin,
        until 2020, kombucha is a growing segment in a crowded   “but I’d like to be an Ottawa export. For Buchipop, this is
        beverage industry. In a food culture increasingly driven by   just the beginning.” — Tyrone Burke



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