Page 43 - phytochemistry II -pharmD general
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Qualitative tests
❑ Alkaloidal solution in HCL +Ca hypochlorite [Ca(ClO)2 ] bleaching powder
→ orange color.
❑ Froehd's reagent:
• Emetine and cephaline + Froehd's reagent:→ dirty green color (the color
with emetine fades by addition of HC1).
• Psychotrine + Froehd's reagent:→ pale yellow color
Extraction and isolation of ipeca alkaloids
Quantitative determination of ipeca alkaloids
• Emetine has been determined by volumetric, spectrophotometric and
• In biological fluids emetine is determined through HPLC .
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