Page 104 - Applied Pharmacognosy

Sample Management

What Must Not Be Injected into the Column??

❑ Substances that dissolve, or chemically react, with the
packing material.

❑ Examples include strong acids or alkalis or highly corrosive
substances. Such substances will destroy the HPLC column

packing materials. How to solve this problem?!!
Sample derivatization is needed to mask
highly reactive functional groups

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