Page 107 - Applied Pharmacognosy
P. 107
Sample Management
Scaling Injection Volume
❑ Sample injection volume is also an important parameters
during HPLC analysis.
❑ Injection volume should be less than 5% of column volume.
❑ Aim for less than 1% and experimentally determine if you can
go higher based on chromatographic conditions.
❑ If you inject too much, the result will be poor peak shape due
to overload.
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