Page 106 - Applied Pharmacognosy

Sample Management

Sample Size

❑ Sample size is a crucial parameter during

HPLC analysis.
❑ Overloading cause poor peak shapes; thus

sample size should be adjusted according to

the column capacity.
❑ Typical values for total amounts of sample in a run are:
✓ Analytical column (25cm x 4.6mm) <100 µg.
✓ Semi-preparative column (25cm x 10mm)

<100 mg. 105
✓ Preparative column (25cm x 21.2mm)

<500mg.