Page 150 - Applied Pharmacognosy
P. 150
Mobile Phase-Elution Types
Gradient elution
❑ Eluent composition is steadily changed during the run to increase
eluent strength and elute more strongly retained solutes.
❑ If one solvent does not provide sufficiently rapid elution of all
components, then Gradient elution can be used.
❑ Increasing amounts of organic solvent B are added to aqueous
solvent A to create a continuous gradient.
Gradient !!!!!!
149
