Page 152 - Applied Pharmacognosy
P. 152
Why Gradient System is Important?
❑ Gradient mode is a way of reducing the analysis time
while maintaining a good level of separation.
Eluent composition with Gradient !!!!!!
a low elution strength
Eluent composition is changed
gradually during analysis...
Separate components Concentration of methanol in eluent 95%
with short retention
times, then……
Eluent composition with
a high elution strength
Separate and elute 30%
components with long
retention times quickly 151
