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Replacement of Eluent

Guidelines for Setting Mobile Phase Conditions

❑ Mutually insoluble solvents        ❑ Aqueous solutions containing
    must not be exchanged directly.      salt and organic solvents must
                                         not be exchanged directly.
           Water
                                            Buffer solution

       2-Propanol                               Water
Hexane
                                     Water-soluble
                                     organic solvent 152
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