Page 162 - Applied Pharmacognosy

HPLC Column

Column Oven Analysis is often performed
at 40 C to 60 C.

❑ It is very important for column temperature control.
❑ if the column temperature increases by heating:

✓ The load pressure decreases due to a decrease in the

viscosity of the eluent.

✓ The retention time becomes shorter.

✓ Resolution improves by hastening diffusion of solutes.

Heating Mechanisms

❑ Air circulation heating type is commonly used. 161
❑ Block heating type: Aluminum block heater.
❑ Insulated column jacket type: Water bath.