Page 165 - Applied Pharmacognosy
P. 165
What is inside the column-The stationary phase
Column Packings in HPLC
What are the optimal criteria for HPLC stationary phase?
❑ Packings are rigid, very fine with particle size 3-10 µm.
❑ Pore size: 70 to 300 (Superficially porous) to minimize band
broadening due to stagnant pools of mobile phase.
❑ High surface area: 50 to 250 m2/g, efficient separation
performance.
❑ Spherical particle shape, produces homogeneous stationary
phase (high bed stability).
❑ High chemical stability.
❑ High load capacity and recovery rates. 164
