Page 160 - Applied Pharmacognosy
P. 160
HPLC Column
What is the best flow rate for your Column?
❑ Flow rate setting range is wide: 0.01 to 10 ml/min.
Column Internal Silica Flow rate Sample
Type
diameter particle (µm) volume/ min size
Narrow bore 2.1 mm 3 or 5 50 – 250 µl <50 µg
Analytical 4.6 mm 3 or 5 0.5 - 1.5 ml <100 µg
Semi preparative 10 mm 5 or 10 <100 mg
Preparative 21.2 mm 3 – 5 ml <500 mg
10 10 - 30 ml
The code information of a HPLC
column is described on Pumps
the label, it contain
column dimensions and 159
packing material.
