Page 253 - Applied Pharmacognosy
P. 253
Methods of derivatization
1 - Silylation:
produces TMS(trimethyl silyl)derivatives (thermostable , volatile)
For alcohols , phenols , carboxyl , amine , amide , SH containing
compounds.
R – AH + (CH3)3 ─Si ─Cl → R – A Si(CH3)3 + HCl
O ─ Si(CH3)3
│
+ R`─ C ═ N ─ Si(CH3)3 → R – A Si(CH3)3 +
bis trimethylsilyl acetamide
O ─ Si(CH3)3 or NH2
│ │
R`─ C ═ NH
R`C=O
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