Page 25 - MNU-PM503 Parasitology practical book

Pharm D- Clinical Pharmacy Program Third Level Parasitology and virology (PM503)

2- Thick Blood Film:

▪ Procedure:

a. Place 2 or 3 drops of fresh blood (without anticoagulant) on a clean slide.

b. Using the corner of a clean slide, mix and spread the drops of blood in a circle

c. Continue stirring for 30 sec. to prevent the formation of fibrin strands that may

obscure the parasites.

d. Allow the film to air dry at room temperature. No need for fixation.

e. Before staining, the films are leaked in phosphate-buffered water for de-

haemoglobinization of RBCs.

f. Films are stained with Giemsa stain.

3- Combination of Thin and Thick Blood Films:

In field surveys, it is helpful to prepare slides with both a thick and a thin blood

films on the same slide.

B- Staining of Blood Films:

The most common stains used are Giemsa and Leishman stains.

Normal blood Picture:

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