Page 24 - MNU-PM503 Parasitology practical book

Pharm D- Clinical Pharmacy Program Third Level Parasitology and virology (PM503)

Advantage of Capillary blood obtained by finger stick:

Capillary blood samples are preferable in parasitic diseases, in which anticoagulants in

the venous blood specimen can interfere with parasite morphology and staining

characteristics.

A- Preparation of Blood Films:

1- Thin Blood Film:

Procedure:

a- Bring a clean spreader slide, held at a 45° angle, toward the drop of blood on the

specimen slide.

b- Wait until the blood spreads along the entire width of the spreader slide.

c- While holding the spreader slide at the same angle, push it forward rapidly and

smoothly.

Thin film to be
examined

d- Fix the smears by dipping them in absolute methanol.

18