Page 22 - MNU-PM503 Parasitology practical book

Pharm D- Clinical Pharmacy Program Third Level Parasitology and virology (PM503)

3- Permanent Stained Smears:

Special staining techniques are used as the modified Zeihl-Neelsen acid-fast stain.

This stain is used to differentiate schistosoma eggs. The egg shells of S. mansoni

and S. japonicum are acid-fast positive, while that of S. haematobium are negative.

III- BLOOD EXAMINATION

◼ The circulating blood is composed of plasma and cells.

◼ The cells are red cells (or erythrocytes), white cells (or leucocytes) and platelets.

◼ Blood cells can be identified in blood films stained with a mixture of basic and

acidic dyes.

◼ Normal white cells are divided into polymorphonuclear leucocytes (or granulocytes)
and mononuclear cells.

There are three types of granulocyte named according to their staining characteristics
in blood films. They are neutrophils, eosinophils and basophils. Mononuclear cells are

divided into lymphocytes and monocytes

• Type of Sample: either

1- Venous blood samples or 2- capillary blood

samples

Venous blood obtained by venipuncture:

• 1. Label collection tubes and slides with the patient’s name and

date and time of collection.

• 2. Clean the site well with alcohol; allow to dry.

• 3. Collect the venous blood in a vacuum tube containing

anticoagulant (preferably EDTA); alternatively, collect the blood

in a syringe and transfer it to a tube with anticoagulant; mix well.

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