Page 17 - MNU-PM503 Parasitology practical book

Pharm D- Clinical Pharmacy Program Third Level Parasitology and virology (PM503)

Laboratory diagnostic techniques

Laboratory diagnosis is a basic step in confirming a clinical diagnosis of parasitic

infection. Different techniques are used for different specimens. Reliability of the

results of an examination depends on the selected technique carried out. These

diagnostic techniques include:

I- Urine examination

II- Stool examination

III- Blood examination

Precautions during preparation of different specimens from different sources:

- Wear personal protective equipment such as gloves, laboratory coats.

- If you have cuts or abrasions on the skin of your hands, cover them with adhesive

dressing.

- Remove gloves and wash your hands after completing any task involving the handling

of biological material

- Decontaminate work surface at least once a day

I-URINE EXAMINATION

Urine is produced by the kidney to maintain constant plasma osmotic concentration;

to regulate pH.

Usual colors are colorless, yellow, amber, less commonly pink, red, and brown.

Usual appearances (opacity) are clear; less commonly turbid, cloudy and opaque,

unless the specimen has remained at room or refrigerated temperatures

MICROSCOPIC EXAM (looking forward cellular element, yeast, parasite)

Two methods are used, simple and centrifugal sedimentation.

1- Simple Sedimentation:

11