Page 18 - MNU-PM503 Parasitology practical book

Pharm D- Clinical Pharmacy Program Third Level Parasitology and virology (PM503)

a. Urine sample is collected into a conical sedimentation glass and is left for 30

minutes to sediment.

b. With the aid of a glass pipette, few drops of the bottom sediment are pipetted

onto a clean slide.

c. Spread the urine, apply a cover slip and examine microscopically.

2- Centrifugal Sedimentation:

a. Random urine sample is collected and well mixed.

b. About 10 ml of urine sample is placed in a centrifuge tube and centrifuged at

1500 rpm for 5 minutes.

c. The supernatant fluid is discarded.

d. A drop of the sediment is placed on a slide, covered by a cover slip and examined

microscopically.

Some parasitic stages can be detected in urine as S. haematobium egg,

2-STOOL EXAMINATION
Stool specimen:

• Examination of fresh specimens permits the observation of motile

trophozoites, but this must be carried out without delay.

• Liquid specimens (which are more likely to contain trophozoites)

should be examined within 30 minutes of passage (not within 30

minutes of arrival in the laboratory!), and soft specimens (which may

contain both trophozoites and cysts) should be examined within one

hour of passage.

• If delays cannot be avoided, the specimen should be preserved in 10

% formalin or PVP (polyvinyl alcohol) to avoid disintegration of the
trophozoites.

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