Page 21 - MNU-PM503 Parasitology practical book

Pharm D- Clinical Pharmacy Program Third Level Parasitology and virology (PM503)

iii- Acid-Ether Sedimentation Technique:

The advantage of acids used in this technique is their ability to dissolve mucus and

other substances resulting in cleaner sediment.

b- Flotation Methods:

In contrast to sedimentation, flotation concentrates the eggs and cysts at the top

because their specific gravity is less than that of the suspending medium.

i- Erlenmeyer’s Method:

ii- Zinc Sulfate Centrifugal Flotation Method:

Some helminthic eggs (operculated and un-fertilized Ascaris eggs) will not float in

zinc sulfate but sink to the bottom. So, if zinc sulfate is the only concentration method

used, both the surface film and the sediment should be

examined.

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