Page 19 - MNU-PM503 Parasitology practical book
P. 19
Pharm D- Clinical Pharmacy Program Third Level Parasitology and virology (PM503)
Sample preparation:
A- Macroscopic Examination:
By Naked eye:
o Tape worm segments as T. saginata and T. solium may be found on or beneath
the stool on the bottom of the collection container.
o Ascaris lumbricoides, E. vermicularis and T. trichiura are occasionally found
on the surface or in the stool.
o Bloody specimens may indicate some parasites, e.g. S. mansoni.
o Soft or liquid stool is highly suggestive of an amoebic infection.
B- Microscopic Examination:
1- Direct Examination:
i- Direct Wet Smear: Wet smear is prepared by using saline and/or iodine as working
solutions.
▪ Procedure:
a. Place one drop of 0.9% Na Cl on the center of a clean slide.
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