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Table 3
Call rates and total intensity for positive controls, negative controls (NCs), and reagent blanks (RBs). Positive control samples
were run multiple times as part of various studies in this validation. The average call rate for negative controls was 60.3%
(standard deviation (SD) = 1.87%), indicating a majority of genotypes are being called for these samples.
Total
Sample Type Sample ID Call Rate (%)
Intensity
99.28 35,099
99.28 44,479
99.27 40,239
NA12878
99.28 39,835
99.27 34,969
99.27 43,451
Positive Control
99.92 37,549
NA24385 99.92 42,925
99.91 42,820
99.91 41,512
NA24631 99.92 38,623
99.92 43,733
NC1 64.15 767
NC2 60.72 791
NC3 60.43 784
NC4 59.19 969
NC5 63.36 1,127
NC6 58.85 965
NC7 59.28 930
Negative Control NC8 59.40 1,016
NC9 58.80 872
NC10 58.10 943
NC11 61.35 1,129
NC12 59.45 1,084
RB1 60.61 1,156
RB2 58.29 1,013
RB3 60.11 829
Developmental Validation of the Illumina Infinium Assay using the Global Screening Array (GSA) on the iScan System for use in Forensic Laboratories