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                            5



                          4.5




                            4




                          3.5
                         Total Intensity (in thousands)  2.5


                            3









                            2




                          1.5



                            1




                          0.5




                            0
                                    NC1         NC2         NC3          NC4         NC5         NC6         NC7          NC8         NC9         NC10        NC11        NC12         RB1          RB2         RB3
                                                                                                                      Sample ID






                     Figure 1
                      The mean total intensities for the 12 negative controls (NC) and three reagent blanks (RB) across all studies performed for this validation.  The mean total intensity is
                     calculated by summing the p95 red and p95 green intensity values from the GenomeStudio analysis. The baseline noise was calculated at 1,400 by taking 3 standard
                     deviations above this mean (958) and rounding up to the next hundred. To be conservative, the intensity threshold was established at three times the baseline (4,200).
                     Baseline and intensity thresholds are marked by solid black lines.



                                               Developmental Validation of the Illumina Infinium Assay using the Global Screening Array (GSA) on the iScan System for use in Forensic Laboratories
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