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 5



 4.5




 4




 3.5
 Total Intensity (in thousands)  2.5


 3









 2




 1.5



 1




 0.5




 0
 NC1  NC2  NC3  NC4  NC5  NC6  NC7  NC8  NC9  NC10  NC11  NC12  RB1          RB2         RB3
 Sample ID






 Figure 1
  The mean total intensities for the 12 negative controls (NC) and three reagent blanks (RB) across all studies performed for this validation.  The mean total intensity is
 calculated by summing the p95 red and p95 green intensity values from the GenomeStudio analysis. The baseline noise was calculated at 1,400 by taking 3 standard
 deviations above this mean (958) and rounding up to the next hundred. To be conservative, the intensity threshold was established at three times the baseline (4,200).
 Baseline and intensity thresholds are marked by solid black lines.



 Developmental Validation of the Illumina Infinium Assay using the Global Screening Array (GSA) on the iScan System for use in Forensic Laboratories
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