Page 59 - Power of Stem Cells- arthritis and regeneration

Theranostics 2018, Vol. 8, Issue 4 913

indicate that BMMSC-EVs are an essential component proteoglycans and type II collagen production by OA
of BMMSC paracrine signaling promoting chondrocytes.

Figure 2. BMMSC-EVs are taken up by chondrocytes derived from OA patients. CFSE labelled EVs derived from TERT-bone marrow MSC were
incubated for 30 min with OA chondrocytes and the uptake of EVs was analyzed by confocal microscopy. Left panel: Representative images of co-localization analyses
of CFSE labelled (green) BMMSC-EVs with late endosomal marker LAMP1 (red) in OA chondrocytes are shown. Arrow heads indicate the co-localization (yellow) of
BMMSC-EVs with LAMP1. Right panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with early endosomal marker EEA1
(red) in OA chondrocytes is shown. Images are representative of 3 independent experiments. Scale bar is 10 µm.

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