Page 8 - MSC & Exosomes in autoimmune

HUCB-Derived MSCs in Canine Cerebral Ischemia 3561

Fig. 10. Light microscopic images of cortex (A1–A3) and basal gan- in neurons (arrowheads in B3), vacuolization from the neuronal loss
glia (B1–B3) of the left hemisphere with infarction. A1: 3100, A2: (within circles in A2,B3), congestion of blood vessels (boxes in
3400, A3: 3400, B1: 3100, B2: 3200, B3: 3400. Light micro- A2,B1), and infiltration of leukocytes (within rectangles in A3,B2).
scopic findings for the left infarcted hemisphere include cytoplasmic Scale bar 5 60 lm. [Color figure can be viewed in the online issue,
eosinophilia (arrows in A2) and poor nuclear staining and karyolysis which is available at www.interscience.wiley.com.]
155.15 6 24.50% in the control group, n 5 5; P < found in the lesion area. After the TTC staining, the
0.05; Table I). infarcted lesion area was visible as an unstained area cor-
responding to the abnormal signals found in the FLAIR
CBC Count MR images at week 4. Lesion areas involved the MCA
territories, basal ganglia, and thalamus (Fig. 8).
The number of WBCs had increased 12 hr after the
infarction in both groups. In the HUCBC group, the Light Microscopic Findings for HE-Stained
number of WBCs peaked at 12 hr and decreased after- Brain Sections
ward. In the control group, the number of WBCs peaked
at day 3 and remained in the normal range afterward In the intact right hemisphere, no remarkable find-
(Fig. 7). There was no significant increase in the number ing was observed except for some vacuolization of neu-
of WBCs after transplantation of HUCB-derived MSCs. rons resulting from the TTC staining procedure (Fig. 9).
Light microscopic findings for the infarcted left hemi-
sphere included cytoplasmic eosinophilia, poor nuclear
Gross Examination and TTC Staining staining and karyolysis in neurons, vacuolization resulting
On gross examination, the infarct area showed dis- from neuronal loss, congestion of blood vessels, and
colored and atrophic features, and necrotic changes were infiltration of leukocytes (Fig. 10).

Journal of Neuroscience Research