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The central DNA flap of HIV-1, created by central initiation and
termination steps during reverse transcription, is necessary for HIV-1
pre-integration complexes (PICs) to enter the host cell nucleus. In the
absence of this central DNA flap, viral DNA nuclear import is severely
impaired at a stage immediately preceding or during the translocation
of HIV-1 DNA through the nuclear pore. Without the third strand DNA
flap, all of the HIV-1 DNA is unable to enter the nucleus and infect
targeted indivisible cells. The lack of a DNA flap leads to a virus that
is almost non-infectious in both divisible and indivisible cells.
Now you know the key determinant of the nuclear import of the
HIV-1 genome into the nucleus of divisible and indivisible cells. 117
Since the central DNA flap is only 99 nucleotides in length and is not a
complete linear strand, it is labeled as a flap. The presence and function
of this central DNA flap within mRNA-based vaccines is highly
problematic.
Its normal biological function is to mediate the folding of the two
linear strands of the cDNA, while incorporating the enzyme integrase.
The central DNA flap ensures that the two strands of complementary
DNA are compact enough to fit across one of the 1000 pores of the
double-layered nuclear membrane.
The so-called SARS-CoV-2 virus, which acts as a vector for 16
genomic fragments of HIV-1 is necessitated by the lack of a fully intact
genomic viral sequence for HIV-1. The partial genetic information of
HIV-1 is embedded in the global genome sequence of SARS-CoV-2
itself. So, wherever the virus goes, so do the 16 genomic fragments of
HIV-1. Where they move, the so-called SARS-CoV-2 virus moves too.
Since this is an intact sequence of mRNA, both in the form of the
virus itself and as a vaccine (both based on mRNA and DNA), it moves
as a unit. Whether entering through the outer plasma of the cell's
membrane or, through the cytosol, inside the double membrane of the
nucleus, the genetic materials remain together.
The copies, known as complementary, are of the entire mRNA

117 Zhang Liguo et al. (2020). SARS-CoV-2 RNA reverse-transcribed and integrated
into the human genome. bioRxiv 2020.12.12.422516; Doi:https://doi.org/10.1101/2020.
12.12.422516/

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