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Spondias pinnata Leaf Extract Gel Improves Wound
Healing in Diabetic Rats
1
Desak Made Widyanthari , Gusti Ayu Ary Antari , I Made Agus Gelgel Wirasuta , Pande Made Nova Armita Sari 4
2
3
1,2 Department of Nursing, Faculty of Medicine, Universitas Udayana, Indonesia
3,4 Pharmacy Study Program, Faculty of Mathematics and Science, Universitas Udayana, Indonesia
Corresponding author: desakwidyanthari@unud.ac.id
INTRODUCTION RESULT DISCUSSION
Diabetes Mellitus (DM) caused impaired wound healing.
Image 1. Identification of Phytochemical Compoundsof Spondias pinnata leaves
The inflammatory process in diabetic wounds will be Administration leaf extract gel of Spondias pinnata
prolonged due to the accumulation of neutrophils and accelerated the wound healing process in diabetic rats
an excessive increase in Reactive Oxygen Species by shortening the inflammatory phase compared to the
(ROS). Protease inhibitors that protect tissue cells and control group. Antioxidants work by delaying,
the extracellular matrix will be damage. Antioxidants will
preventing, or eliminating oxidative damage to target
inhibit the work of proteases and ROS formed from the molecules. Flavonoids work as ROS scavengers by
accumulation of neutrophils in the wound area and scavenging free radicals, chelating metals, suppressing
protect protease inhibitors from oxidative damage. Leaf enzymes associated with free radical formation and
extract gel of Spondias pinnata (L.f) Kurz contain stimulating internal antioxidant enzymes. Flavonoids are
flavonoids, phenols and alkaloids that suppress ROS Image 2 . Wound healing progress able to inhibit the expression of nitric oxide,
activity through antioxidant and anti inflammatory cyclooxidase and lipoxygenase synthesis isoforms,
activities. This study aims to determine the effect of which are responsible for the inflammatory process,
ethanolic extract gel from Spondias pinnata leaves on resulting improve of wound healing process.
the healing process of diabetic wounds.
CONCLUTION
.
Extract gel of Spondias pinnata leaf significantly
METHODS improves the healing process of diabetic wounds.
ANKNOWLEDGMENTS
The research design was a true experimental research The funding source of the research is from
with randomized post test only control group design. Universitas Udayana with Contract Number
Rats were induced using streptozotocin (STZ) at a dose B/4969-57/UN14.2.2.VII.1/PT.01.03/2021
of 35 mg/kgBB in citrate biffer pH 4.5 intraperotoneally. Reference
A total of 29 male rats which were divided into 5 groups;
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Antioxidants and Natural Products in Inflammation. Oxidative Medicine and Cellular Longevity, 2016.
given leaf extract gel of Spondias pinnata with a https://doi.org/10.1155/2016/5276130
concentration of 5%, 7.5% and 10%. The wound was • Azizah, S., Nursamsiar., & Nur, S. (2019). Uji Aktivitas Antioksidan Ekstrak Etanol Daun Kedondong Hutan (Spondias
pinnata (L.F.) Kurz.) Dengan Berbagai Metode Uji. J. Akademika Kim., 5(1), 91–96.
made using a 5 mm puch biopsy on the back. Spondias • Ebaid H, Ahmed O, Mahmoud A, Ahmed R. (2013). Limiting prolonged inflammation during proliferation and remodeling
phases of wound healing in streptozotocin-induced diabetic rats supplemented with camel undenatured whey
pinnata leaf extract gel was applied twice a day for 10 protein. BMC Immunol. 14:31
• Sujarwo, W., Saraswaty, V., Keim, A. P., Caneva, G., & Tofani, D. (2017). Ethnobotanical uses of ‘cemcem’ (Spondias
days. pinnata (L. F.) kurz; anacardiaceae) leaves in bali (Indonesia) and its antioxidant activity. Pharmacologyonline, 1(July),
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• Süntar, I., Akkol, E. K., Nahar, L., & Sarker, S. D. (2012). Wound healing and antioxidant properties: do they coexist in
plants? Free Radicals and Antioxidants, 2(2), 1–7. https://doi.org/10.5530/ax.2012.2.2.
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4 Udayana International Nursing Conference
