Page 68 - Practical Technology 2025

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Clinical Pharmacy-Pharm D Level Three 2 Semester 2024/2025 Pharmaceutical Technology (PT 607)

Example 1:
Use the following calibration curve to do find out the drug concentration.

• 1 ml of each sample was diluted to 10 ml with 0.1N HCl
• Drug content = 100 mg
• Volume of Dissolution medium = 500 ml

0.5
y = 430x+ 0.0083
0.45
0.4
R² = 0.999
0.35
0.3
Absorbance 0.15
0.25
0.2

0.1
0.05
0
0 2 4 6 8 10
Conc. (mg/ml)

Time (min) Absorbance Concentration (mg/ml) % Drug Dissolved
0 0
15 3.5
30 4.55
45 5.54
60 5.98
90 6.8
120 8

Example 2:
Use the following calibration curve to do find out the drug concentration.

• Drug content = 100 mg

Calibration curve in 900 ml 0.1N HCl
1.2

1
Absorbance 0.8

0.6

0.4
0.2

0
0 0.01 0.02 0.03 0.04 0.05 0.06
Conc. (gm %)

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