Page 48 - Бог малын сохор догол өвчнөөс сэргийлэх идэвхгүйжүүлсэн вакцины загвар бэлтгэн сорьсон дүн

Бог малын сохор догол өвчнөөс сэргийлэх идэвхгүйжүүлсэн вакцины загвар 2013
бэлтгэн сорьсон дүн

Overall goal of this study:

The present study aimed to develop a safe inactivated vaccine against

contagious agalactia and evaluation it’s efficacy.

Objective of this study:
In order to accomplish overall goal, the below mentioned objectivies were put

forward. These are as follows:

To evaluate an epidemiological situation of contagious agalactia in Mongolia,
using statistical data from Governmental Agency of Veterinary and Animal Breeding.

To isolate causative local agents of contagious agalactia of small ruminants in
Mongolia and identify the causative agents of contagious agalactiae of small

ruminants by cultural, morphological and biochemical properties and characterization

by PCR
To select vaccine candidate strains of M. agalactiae and develope a phenol

inactivated vaccine and evaluate it’s efficacy.

According to statistical data from Governmental Agency of Veterinary and
Animal Breeding, contagious agalactia has been occurring every year in Western and

Central regions of Mongolia, particularly in Uvurkhangai, Bayankhongor, Zavkhan,

Govi-Altai, Uvs, Khovd, Dundgovi aimags. But distribution are of this infection seems
like expanding, by our study we had isolated M. agalactiae from goat the in Darkhan

suom, Khentii province, which were free from this infection previously. This infected
flock was moved to this province from Uvs aimag which is an endemic with this

infection.

As a result of this study, we had been isolated 37 mycoplasma like agents from
samples, collected from goat with clear clinical symptoms and had been characterized

as the M. agalactiae by PCR.
All isolates were positive by standart PCR with M. agalactiae specific primers,

developed by previously foreign researchers and recommended by OIE for

identification of causative agents of this infection.
Also, we had performed the partial sequencing of the UrvC gene of isolates and

compared with reference strains. Our results suggested that, all Mongolian isolates

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