Page 50 - Шувууны ньюкасл, бронхит өвчний хам вакцины дархлааны хөдлөлзүйг судалсан нь

Study of immunological dynamics of dual vaccine against avian Newcastle
disease and Infectious bronchitis

Although the number of business people engaged in intensified farming are
increasing in the last years, such activities as importation of a day old hatching eggs
together with the vaccine by poultry farms, which are active in our country, from
Russian Federation and Peoples Republic of China, incubation of eggs for
producing viable chicks, formation of new flocks of layers, rearing of their chicks, and
vaccinations of them in fixed time are considered to be enormously dangerous for
countries similar to our country, where issues of diagnosis of highly contagious
diseases in birds have not been fully solved. For our country, it is highly probable
that some infectious diseases (Marek’s disease, laryngotracheitis etc), which are not
reported or diagnosed or impossible to be diagnosed nowadays can be introduced
via importation of new flocks for restocking. If cases of any diseases occur,
enormous loss will be caused in our country with lower economic capacity.

This research was carried out relying on “Tumen Shuvuut” LLC by making the
contract of collaboration.

The poultry farm involved in the current study imports fertile or embryonated eggs
from both Russian Federation and PRC in order to form breeder flock, then places
them in incubator for 21 days for producing viable chicks, then sexing and sorting a
day old chicks and rearing female chicks, which will become layers. Vaccinations are
made according to scheduled time. It was planned to perform the study on
immunological dynamics of dual vaccine against avian Newcastle disease and
infectious bronchitis in this poultry flock for the life span or period from a day old
chick to laying hen. To achieve this goal the following objectives were put forward:

Objectives:

- Blood samples were collected from male chicks before the vaccination;
- Blood samples were collected in fixed time and dynamics of antibodies produced in
the blood was determined after the scheduled immunization of chicks by using dual
vaccine against avian Newcastle disease and infectious bronchitis;

-Sterility of both Newcastle disease and infectious bronchitis viruses to be used for
Hemagglutination Inhibition (HAI) test was tested, and titrations were determined by
culture in embryonated egg;

-ELISA test was used in order to confirm the results of HAI test.

Conclusions
1.Testing of Newcastle disease and infectious bronchitis virus contamination by
culturing on SCD and TG media and confirmation of freedom from contamination
has been important for the present study. Because death of emryonated egg due to
bacterial or fungal contaminations or ineffectiveness can occur during culture of virus

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