Page 51 - Шувууны ньюкасл, бронхит өвчний хам вакцины дархлааны хөдлөлзүйг судалсан нь

in embryonated egg, the study revealing the feasibility of its prevention becomes
less money and time consuming and effective work.

2.Newcastle disease and infectious bronchitis viruses were cultured in embryonated
egg, materials were harvested, titrations were determined by hemagglutination test
and a total of 900 serum samples collected from chicks for pre and post vaccination
periods were tested by HAI test. The testing results show 100 control samples had
high titrations, samples for Newcastle disease virus had titrations between 1:256 and
1:512, while infectious bronchitis virus had titrations from 1:32 to 1:64. Above
demonstrates chicks have maternally transmitted immunity. Because this maternal
immunity decreases from day 3, dual vaccine against Newcastle disease and
Infectious bronchitis was used during this period. Repeated vaccinations were made
at days 21 and 90.

3.Blood samples were taken from 1, 2 and 3 months old chicks and 9, 12, 18, 21
and 24 months old laying hens after the vaccination, serum was isolated from blood
and the serum samples were tested by determination of dynamics of antibodies
produced in the blood. Antibody titrations in blood samples taken at months 1, 2, 3, 9
and 12 for both Newcastle disease and Infectious bronchitis ranged from 1:128 to
1:512 and from 1:32 to 1:64 respectively. It was also found that antibody titrations in
blood samples taken at months 15, 18 and 24 for both Newcastle disease and
Infectious dropped ranging between 1:16 and 1:128 and 1:4 and 1:64 respectively.

4.Because the blood antibody body titrations ranging from 1:320 to 1:640 and from
1:80 to 1:160 for Newcastle disease and Infectious bronchitis virus at day 0 by
ELISA testing were also higher similarly to HAI test results, it demonstrated the chick
had maternally transmitted immunity. Although dynamics of antibodies produced in
the blood taken at month 1 after the vaccination were from 1:640 to 1:1280 for
Newcastle disease virus and from 1:160 to 1:320 for Infectious bronchitis virus,
antibody titrations at month 2 after the vaccination increased ranging from 1:1280 to
1:2560 and from 1:160 to 1:320 respectively. At months 3 and 9, antibody titrations
for Newcastle disease virus were constant at 1:1280. Antibody for bronchitis virus in
the blood ranged between 1:80 and 1:160. Titrations for Newcastle disease and
Infectious bronchitis virus at months 18 and 21 were from 1:160 to 1:320 and from
1:40 to 1:80 respectively, while those at month 24 ranged from 1:40 to 1:80 and from
1:20 to 1:40 respectively.
5.It has been demonstrated that chicks survived by incubation of embryonated eggs,
which are imported by our country poultry farms from foreign countries have
maternally transmitted immunity for the first 3 days of their life.

6.It has been proved that vaccines imported together with eggs and chicks and
injected thereafter provide lifelong protection against both Newcastle disease and
Infectious bronchitis.

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