Page 52 - Abstract Book ICOH4

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Antibiotic Resistance of Staphylococcus Coagulase Negative Causes Subclinical Mastitis In
Lucky Farm, Medowo Village, Kandangan District, Kediri Regency

ABSTRACT

Mastitis is an inflammation of the mammary gland, can be occurred clinically or subclinically. One of the
causes of mastitic is Staphylococcussp. It is often caused by improper treatment with antibiotics that can lead to
bacterial resistance. This study aims to isolate and identify Staphylococcus sp. and to determine the sensitivity
toward Ciprofloxacin, Gentamicin, Tetracycline, Cefadroxil, and Ampicillin. This study used 25 samples of milk from
Lucky farm and then will be tested later with the mastitic test using Californian Mastitis Test (CMT). The CMT positive
sample +1 until +4 will be inoculated in Tryptone Soya Broth (TSA) and Mannitol Salt Agar (MSA) and incubated
24 hours at 37⁰C. The colonies which grew on the plate is identificated based on the morphology, and followed by
gram staining, catalase test, and coagulase test to confirm the species of the bacteria. A sensitivity test will be done
by taking the colony of identified bacteria in Mannitol Salt Agar (MSA) and then will inoculate on the Nutrient Agar
(NA) to grow the bacteria. The colonies will equalize with 0,5 Mc Farland 1,5 x 108 CFU/mL standard and will be
inoculate on Muller Hinton Agar (MHA). Place the Penicillin G, Ciprofloxacin, Gentamicin, Tetracycline, Cefadroxil and
Ampicillin paper discs on Muller Hinton Agar (MHA). The results from isolation and identification of the bacteria
caused subclinical mastitis, S.aureus bacteria as much as 33,33%, and 66,66% Staphylococcus coagulase negative
(CNS). The results of the highest percentage of antibiotic resistance test are Penicillin G (67%), Tetracicline (67%)
and Ampicillin (33%) on S.aureus. The results of the antibiotic resistance test of Penicillin G (33%), Cefadroxil (17%),
and Ampicillin (17%) on CNS bacteria. The antibiotic recomedation for Lucky Farm are quinolones and
aminoglycosides which are still sensitive to S.aureus and CNS bacteria.

Key words: Subclinical mastitis, Staphylococcus aureus, Antibiotic resistance, Diary farm, Kediri

THE 4 INTERNATIONAL CONFERENCE ON ONE HEALTH (ICOH)
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“Strengthening Collaboration in One Health Systems”