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DETECTION OF Streptococcus suis SEROTYPES 2 AND 9 IN TONSILS AND LUNGS OF PIGS

Tee Jia Hui, Lee Chee Yien,
1Gayathri Thevi Selvarajah & 1Ooi Peck Toung
1Department of Veterinary Clinical Studies
Faculty of Veterinary Medicine
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
*Correspondence: ooi@upm.edu.my

ABSTRACT

Streptococcus suis is a Gram-positive bacterium under Streptococcaceae family that acts as a natural
inhabitant in the porcine upper respiratory tract. It causes inflammatory diseases in pig, mainly in weanlings,
characterised by septicaemia, meningitis and pneumonia. The isolation of S. suis from dogs, cats, ruminants
and horses has also been previously reported, indicating S. suis has a broad host range. In Asia, it has emerged
as a significant cause of bacterial meningitis in human, and becomes a major public health concern particularly
with regard to the occupational risks as well as food-borne related transmission. To control this problem in
swine industry, serotyping of S. suis is crucial for effective local vaccine development and also understanding
its pathogenesis. In addition, as cat is an important companion animal with close attachment to humans, there
will be significant benefits in detection and prevention of this zoonotic disease, if specific serotypes of S. suis
is able to be identified in cats. Therefore, the objectives of this study were to detect S. suis and also to amplify
serotypes 2 and 9 of S. suis in pig tissue and cat serum samples using S. suis detection (glutamate
dehydrogenase gene) PCR and serotype-specific primer PCR assays. A total of 30 porcine archived tonsil
tissues and 30 porcine lung tissues were extracted for DNA. DNA from a total of 40 archived serum samples
from cats, which consist of 20 stray and 20 pet were also extracted. All samples were subjected to the S. suis
detection PCR followed by detection of serotypes 2 and 9 using the serotype-specific primer PCR assay for only
S. suis positive samples. In pig tissue samples, 41.67% (25 of 60 samples) were positive for S. suis, and
serotype 9 (8.33%) was detected higher than serotype 2 (1.67%). Meanwhile, none of the serum samples
obtained from both groups of cats were positive for S. suis.

Keywords: Streptococcus suis, pig, cat, molecular serotyping.

TH
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