Page 327 - 00. Complete Version - Progress Report IPEN 2014-2016

Materials and Nanotechnology | Progress Report 327

Figure 51. Fluorescence spectroscopy of bovine serum albumin biding to gold nanoparticles

We have also explored the chromophores of biological affinity with a considerable lack of
Albumin as a possible internal dosimeter for toxicity when administered to humans. With-
radiation therapy as follow bellow (Figure 51). in this context, several studies have been per-
formed using proteins as nanocarriers. In this
Development of protein based work, we developed protein based nanocarri-
nanocarriers for the delivery ers, using bovine serum albumin or papain
of radiopharmaceuticals as model protein and enzyme respectively, by
the use of radiation. Unlike conventional path-
Nanoparticles and nanoparticulate systems ways, radiation allows the sterilization and
are considered a breakthrough in several fields simultaneous crosslinking of the protein in a
as novel applications and features of common- single step, without the need for toxic mono-
ly used materials were brought to light as a re- mers or crosslinkers that commonly lead to
sult of the nanostructuration. Biopharmaceu- undesirable reactions when administered to
tical and biomedical advantages, including biological systems. The nanocarriers are being
site specific delivery are good examples of ad- radiolabeled with 99mTc by the direct and in-
vantages that are offered by nanotechnology direct method, in order to achieve an alterna-
over conventional systems. At the same pace, tive system for the delivery of this and oth-
concerns regarding toxicity emerge as most er radioisotopes for diagnose and treatment
molecules or nanoparticles have no biologi- of a variety of disorders. High radiolabeling
cal affinity, thus adverse reactions may take yields with good radiochemical stability have
place including non-specific toxicity. Within been identified throughout the experiments
this context, the seek for biologically-friend- and further steps concern the assessment of
ly materials are of highlighted role. Proteins ex vivo and in vivo biodistribution of the de-
are biocompatible by nature and hold strong veloped systems.