Page 144 - Feed Lab

Suguna Management System Ver 1.0 / SOP / FQL / P3 - 08 Page 1 of 1

8. Determination of Cotton Seed Oil in other Oils

SCOPE PROCEDURE
Applicable to test of all type of Oil. FTake 5.0 ml of the oil in clean and dry test tube.

FAdd 5 ml of Sulfur Solution .Shake vigorously and
OBJECTIVE
heat gently in a water bath (70- 80ºC) for few
This procedure is for the determination of Cotton Seed minutes with occasional shaking until the carbon
Oil in Soya Crude oil.
disulphide has boiled off and sample stops
foaming, Place the tube in oil bathe or saturated
APPARATUS
brine bath maintained 110-115ºC for 2 hrs.
FTest tube,
FThe presence of Cotton Seed Oil indicates by Red
FWater Bath, Colour in the solution.

FOil Bathe etc.
REFERENCE:
IS 548(Part II)-1976.
REAGENTS
Sulphur Solution: Prepare a one percent (m/v)
solution of sulphur in carbon di sulphide and then add
an equal volume of Amyl Alcohol.

Prepared by : Approved by :
130 Director - Technical