Page 140 - Feed Lab

Suguna Management System Ver 1.0 / SOP / FQL / P3 - 05 Page 1 of 1

5. Determination of Plant Protein

drops of chlor-zinc iodine solution and let stand for
PRINCIPLE
10 min. (Picture - 3)
Carbohydrates from plants contain starch and
cellulose. When it reacts with iodine and chlor-zinc FA purple brown color indicates the presence of
iodine solution, the starchy tissue releases a blue color plant fiber, whereas yellow indicates animal fiber
and the plant fiber or cellulose develops a purple- (protein) using a microscopic examination.
brown color when examined under a microscope. (Picture - 4)

REAGENTS
FIodine solution: 0.5 g I and 1.5 g KI dissolved in 25
ml distilled water.

FChlor-zinc-iodine solution: Dissolve 100 g ZnCl
2
in 60 ml water, and then add 20 g KI and 0.5 g I.

PROCEDURE I
Picture - 1 Picture - 2
FMix 1-2 g test sample with 100 ml boiling water or
boil the mixture for 2-3 min and filtrate the solution.
(Picture - 1)

FPlace a few ml of the cooled mixture on a spot plate
or test tube and add 5-6 drops of iodine solution. If
starch is present, the mixture turns blue.
(Picture - 2)

PROCEDURE II
FSpread 1-2 g test sample into a Petri dish. Add 5-6 Picture - 3 Picture - 4

Prepared by : Approved by :
126 Director - Technical