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The 4 Educational Science International Conference
Towards a Sustainable Future of Education: Preparing Teachers and Prospective Teachers for the Digital Age

SCIED-02

Antioxidant Activity And Antiproliferation of HeLa Cells From Methanol Extract of
Leaves And STEM Bark of Mangrove Avicennia marina

Usman, Aisyah Fitri, Linawati, Nirmala Trisna
Mulawarman University, Indonesia
sainusman@ymail.com

Abstract

Indonesia stores thousands of species of medicinal plants that can be utilized. One of the
plant species that has efficacy as an alternative medicinal ingredient is the mangrove plant
Avicennia Marina. The purpose of this study was to find out the content of secondary
metabolite compounds, antioxidant activity and antiproliferation activity of HeLa cells
contained in methanol extract of leaves and bark of mangrove stems A. marina. This study
was conducted 3 tests, namely phytochemical tests, antioxidant tests and antiproliferation
tests. Phytochemical tests are conducted with color tests, these results showed methanol
extract of mangrove leaves A. marina contains alkaloid compounds, flavonoids, steroids and
tannins while methanol extracts mangrove stem A. Marina contains alkaloid compounds,
flavonoids, triterpenoids and tannins. The antioxidant activity test is done using the
DPPH method. The results of antioxidant activity test samples of A. Marina leaf methanol
extract had an IC value of50 of 71.13 ± 0.48064 ppm and stem skin methanol extract. A.
marina amounted to 54.023 ± 1.5843 ppm. The results of antiproliferation activity tests
showed an IC value of50 A leaf methanol extract. marina amounted to 618.32 and
methanol extract stem skin A. marina amounted to 305.44. Based on this, the antioxidant
activity of A. marina leaf methanol extract and A. marina stem skin methanol extract is
classified as strong and antiproliferation activity of A leaf methanol extract. Marina is
classified as less active while methanol extract stem skin A. Marina is quite active as a
cytotoxic agent.

Keywords: Avicennia marina, Fitochemical, Activity Antioxide, Antiproliferasi

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