Page 89 - Botany
P. 89
1. Pviv Drcv`b t DbœZ I Dbœqbkxj A‡bK †`‡kB AvRKvj dyj, dj ev km¨ Drcv`bKvix †h‡Kvb DbœZ Rv‡Zi Dw™¢` †_‡K wUmy¨
KvjPvi cÖhyw³i gva¨‡g evwYwR¨Kfv‡e AwaK msL¨K Pviv ˆZwi Kiv n‡”Q| †h‡nZz Gme Pviv mivmwifv‡e ˆ`wnK wUmy¨ †_‡K Drcbœ
nq, ZvB G¸‡jv ûeû G‡`i gvZ… Dw™¢‡`i ¸Ym¤úbœ n‡q _v‡K|
2. msKi Dw™¢` Drcv`‡bi †¶‡Î ‡cÖv†Uvcøv÷ wgjb t G c×wZ cÖ‡qvM K‡i `ywU wfbœ cÖRvwZi †cÖv‡Uvcøv÷ mshyw³ I Zv †_‡K bZzb
ˆewkó¨m¤úbœ msKi Dw™¢` Drcbœ Kiv m¤¢e n‡”Q| Giyc wUmy¨ KvjPvi cÖhyw³‡Z mvaviYZ †K¬v‡ivcøv÷, gvB‡UvKwÛªqv I ivB‡ev‡mvgxq
ˆewkó¨mg~‡ni mgš^q NwU‡q bZzb Rv‡Zi Dw™¢` Drcv`b Kiv n‡”Q|
3. å~Y KvjPvi cÖhyw³i gva¨‡g Dw™¢‡`i K…wÎg cÖRbb t msKi Dw™¢` RvZ D™¢ve‡bi Rb¨ å~Y KvjPvi cÖhyw³i gva¨‡g å~Y D×vi K‡i Zv
cieZx©‡Z M‡elYvMv‡i Avev` Kiv n‡q _v‡K| we‡kl K‡i AvšÍtcÖRvwZK msK‡ii åƒY wVKfv‡e weKvk jvf K‡i bv e‡jB Giyc
åƒY‡K KvjPvi K‡i dm‡ji RvZ Dbœqb m¤¢e|
4. n¨vcø‡qW jvBb t civM‡iYy I civMavbx KvjPvi K‡i Dw™¢‡`i n¨vcø‡qW jvBb cÖwZôv K‡i Zv †_‡KB mn‡RB AvKvw•¶Z wWcø‡qW Dw™¢` cvIqv m¤¢e nq|
5. ‡gwi‡÷g KvjPvi t †gwi‡÷g wUmy¨‡K KvjPvi K‡i †ivMgy³ (we‡kl K‡i fvBivmgy³) Dw™¢` cvIqv m¤¢e n‡q‡Q| D`vniY Ñ U‡g‡Uv, wkg BZ¨vw`|
6. Dw™¢` msi¶Y t Dw™¢` msi¶YK‡í, we‡kl K‡i jyßcÖvq I weij Dw™¢` cÖRvwZ‡K wejywßi nvZ †_‡K i¶v Kivi Rb¨ AvRKvj wUmy¨
KvjPvi cÖhyw³ mdjfv‡e e¨eüZ n‡”Q|
7. ‡Kvl Avev` I K¨vjvm wUmy¨ Avev` t GB cÖhyw³i gva¨‡g GKw`‡K ‰`wnK åƒY I Ab¨w`‡K AYyPviv I †mvgv‡K¬vbvj f¨vwi‡qkb Drcbœ
Kivi be w`MšÍÍi m~Pbv n‡q‡Q| ˆ`wnK åƒY †_‡K K…wÎg exR Drcv`b I †mvgv‡K¬vbvj f¨vwi‡qkb‡K Kv‡R jvwM‡q DbœZ RvZ †hgb
Mg D™¢veb Kiv n‡q‡Q|
N) wUmy¨ KvjPvi cÖhyw³i eY©bv t wb‡gœ wUmy¨ KvjPvi c×wZ eY©bv Kiv n‡jv Ñ
1) Explants wbe©vPb t †h Dw™¢` †_‡K explant (tissue culture Gi Rb¨ e¨eüZ Dw™¢`vsk) wbe©vPb Kiv n‡e †m Dw™¢`wU
¯^v¯’¨evb, wb‡ivM I fvBivmgy³ n‡Z n‡e|
2) Culture media t Explant Gi cywó Pvwn`vi Dci wfwË K‡i mvaviYZ culture media wbe©vPb Kiv nq| Culture
media wewfbœ cÖKvi n‡Z cv‡i| Z‡e me culture media -‡ZB †gŠwjKfv‡e A‰Re jeY, carbohydrate, vitamin,
hormone Ges cvwb _v‡K| A‡c¶vK…Z KwVb gva¨‡g Bnv‡`i mv‡_ agar _v‡K|
3) RxevYygy³KiY t GB ch©v‡q cÖ¯‘ZK…Z Culture media ‡K KwbK¨vj dv¯‹ ev †U÷ wUD‡e †X‡j autoclave Gi gva¨‡g
Rxevbygy³ Kiv nq| wgwWqvg‡K A‡Uv‡K¬f hšÍ¿ wbw`©ó Zvc (121 †m.), Pvc (15 cvDÛ) I mgq 20 ivLv nq|
0
4) wgwWqv‡g explant ¯’vcb t explant ‡K wbRx©e K‡i di‡m‡ci gva¨‡g Rxevbygy³ media ‡Z ¯’vcb Kiv nq|
5) K¨vjvm m„wó I msL¨ve„w× t
wgwWqv‡g explant ¯’vc‡bi ci
cvÎwU‡K GKwU ˆe`y¨wZK Av‡jv
(3000-5000 jv·) I ZvcgvÎvi
(17-20 c) wbqwš¿Z K‡¶ ivLv
0
nq| K‡qKw`b ci explant
Av‡¯Í Av‡¯Í callus (Awbqwš¿Z
wefvRb¶g †Kvl wcÛ ) G cwiYZ
n‡e| Callus ‡_‡K GKmgq
AmsL¨ AbyPviv †ei n‡e|
6) Pviv Drcv`b t m„ó AbyPviv¸‡jv mveav‡b †K‡U wb‡q rooting medium (auxin mg„×) G †`Iqv nq root m„wói Rb¨| wKQy
w`b ci root m„wó nq Ges c~b©v½ Pviv cvIqv hvq|
7) Pviv Dw™¢‡`i ¯’vbvšÍi t c~b©v½ Pviv¸‡jv cÖ_‡g gvwU fiv cv‡Î mveav‡b ¯’vbvšÍi K‡i gv‡S gv‡S cÖvK…wZK cwi‡e‡k K‡¶i evB‡i
†i‡L mnbxq K‡i †Zvjv nq| Pviv¸‡jv mRxe I mej nq DV‡j gv‡V ¯’vbvšÍi (Acclimatization) Kiv nq|
2| cøvRwgW Kx? Gi ˆewkó¨ wjL|
cøvRwgW t cøvRwgW n‡jv extra chromosomal DNA hv e„ËvKvi Ges AvZ¥cÖRbbkxj| cøvRwg‡Wi ˆewkó¨ wb‡P †`Iqv n‡jv-
0 1 7 1 3 7 4 7 2 3 5 2
