Page 176 - Essential Haematology

162 / Chapter 11 Haematological malignancy: aetiology and genetics

Cell sample
RNA extraction

Fluorescent
labelling of RNA

Hybridization
cDNA or
oligonucleotide
microarray

Fluorescent detection
by laser scanning
Read-out of
fluorescence on
microarray
(a)
ALL AML
Genes highly expressed in ALL
C-myb (U22376) Figure 11.13 (a) Principle of

Proteasome iota (X59417)
MB-1 (U05259) transcriptional profi ling of RNA
Cyclin D3 (M92287)
Myosin light chain (M31211) expression in leukaemia samples
RbAp48 (X74262)
SNF2 (D26156)
HkrT-1 (S50223) using DNA microarrays.
E2A (M31523)
Inducible protein (L47738) (b) Microarray analysis of genes
Dynein light chain (U32944)
Topoisomerase II β (Z15115) distinguishing acute lymphoblastic
IRF2 (X15949)
TFIIEB (X63469) leukaemia (ALL) from acute
Acyl-coenzyme A dehydrogenase (M9143)
SnF2 (U29175) myeloid leukaemia (AML). The 50
(Ca2+)–ATPase (Z69881)
SRP9 (U20998) genes most highly correlated on
MCM3 (D38073)
Deoxyhypusine synthase (U26266) gene - expression microarrays with
Op 18 (M31303)
Rabaptin-5 (Yo8612) each of these leukaemias are
Heterochromatin protein p25 (U35451)
IL-7 receptor (M29696) shown. Each row corresponds to
Adenosine deaminase (M13792)
Genes highly expressed in AML a gene; each column corresponds
Fumarylacetoacetate (M55150)
Zyxin (X95735) to the expression value in a
LTC4 synthase (U50136)
LYN (M16038) particular sample. Expression for
HoxA9 (U82795)
CD33 (M23197) each gene is normalized across
Adipsin (M84526)
Leptin receptor (Y12670) the samples such that the mean
Cystatin C (M27891)
Proteoglycan 1 (X17042) is 0 and the SD is 1. Expression
IL-8 precursor (Y00787)
Azurocidin (M96326) greater than the mean is shaded
p62 (U46751)
CyP3 (M80254)
MCL1 (L08246) in red, and that below the mean is
ATPase (M62762)
IL-8 (M28130) shaded in blue. Although the
Cathepsin D (M63138)
Lectin (M57710) genes as a group appear
MAD-3 (M69043)
CD11c (M81695) correlated with the type of
Ebp72 (X85116)
Lysozyme (M19045) leukaemia under study, no single
Properdin (M83652)
Catalase (X04085) gene is uniformly expressed
across the class, illustrating the
SDs above or below mean
–3·0 –2·5 –2·0 –1·5 –1·0 –0·5 0 0·5 1·0 1·5 2·0 2·5 3·0 value of a multigene prediction
Low High
Normalised expression method. (Reproduced courtesy of
(b) Golub and colleagues.)