Page 273 - parasitology for medical and clinical laboratoryprofessionals

CHAPTER

12

Laboratory Procedures

for Identifying Parasitic

Organisms and Their Ova

LEARNING OBJECTIVES

Upon completion of this chapter, the learner will be expected to:

■ Relate the important components of properly collecting specimens to include blood, feces, and
sputum in order to enhance the recovery rate for parasites present
■ Explain to a simulated patient by role-play the steps involved in collecting and safely transporting
a specimen to the clinical laboratory
■ Discuss the theory for making both thick and thin smears for blood pathogens
■ List the four species of malaria that are common human pathogens
■ Describe the symptoms of malaria and how the fever cycles vary among species
■ Prepare and stain both thick and thin smears
■ Discuss the necessity of safety precautions in using blood specimens for parasite studies
■ Contrast the two hematological stains, Wright and Giemsa, and when each are used
■ Use tables, images, and narrative to identify parasites in a variety of specimens

KEY TERMS

Axoneme Gametocytes Sigmoidoscope
Coplin jars Gomori stain Supernatant
Crustaceans Indirect method Thin smear
Direct smear Malarial stippling Trichrome
Duodenal aspirates Meningoencephalitis Wet mount
EDTA Mortality Zinc sulfate
Ethyl acetate Ocular units Zygote
Flotation Schizont
Formalin Sedimentation

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