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CAMBRIDGE UNIVERSITY PRESSCambridge, New York, Melbourne, Madrid, Cape Town, Singapore,Sa%u02dco Paulo, Delhi, Dubai, TokyoCambridge University PressThe Edinburgh Building, Cambridge CB2 8RU, UKPublished in the United States of America byCambridge University Press, New Yorkwww.cambridge.orgInformation on this title: www.cambridge.org/9780521516358First and second editions#Bryan Williams and Keith Wilson 1975, 1981Third edition#Keith Wilson and Kenneth H. Goulding 1986Fourth edition#Cambridge University Press 1993Fifth edition#Cambridge University Press 2000Sixth edition#Cambridge University Press 2005Seventh edition#Cambridge University Press 2010This publication is in copyright. Subject to statutory exceptionand to the provisions of relevant collective licensing agreements,no reproduction of any part may take place withoutthe written permission of Cambridge University Press.First published by Edward Arnold 1975 asA Biologist%u2019s Guide to Principles and Techniques of PracticalBiochemistrySecond edition 1981; Third edition 1986Third edition first published by Cambridge University Press 1992; Reprinted 1993Fourth edition published by Cambridge University Press 1994 asPrinciples and Techniques of PracticalBiochemistry; Reprinted 1995, 1997; Fifth edition 2000Sixth edition first published by Cambridge University Press 2005 asPrinciples and Techniques of Biochemistryand Molecular Biology; Reprinted 2006, 2007Seventh edition first published by Cambridge University Press 2010Printed in the United Kingdom at the University Press, CambridgeA catalogue record for this publication is available from the British LibraryLibrary of Congress Cataloging-in-Publication DataPrinciples and techniques of biochemistry and molecular biology / edited by Keith Wilson,John Walker. %u2013 7th ed.p. cm.ISBN 978-0-521-51635-8 (hardback) %u2013 ISBN 978-0-521-73167-6 (pbk.)1. Biochemistry%u2013Textbooks. 2. Molecular biology%u2013Textbooks. I. Wilson, Keith, 1936%u2013 II. Walker,John M., 1948%u2013 III. Title.QP519.7.P75 20096120.015%u2013dc22 2009043277ISBN 978-0-521-51635-8 HardbackISBN 978-0-521-73167-6 PaperbackCambridge University Press has no responsibility for the persistence oraccuracy of URLs for external or third-party internet websites referred toin this publication, and does not guarantee that any content on suchwebsites is, or will remain, accurate or appropriate.