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7.3 Immunoassay formats 2837.4 Immuno microscopy 2917.5 Lateral flow devices 2917.6 Epitope mapping 2927.7 Immunoblotting 2937.8 Fluorescent activated cell sorting (FACS) 2937.9 Cell and tissue staining techniques 2947.10 Immunocapture polymerase chain reaction (PCR) 2957.11 Immunoaffinity chromatography (IAC) 2957.12 Antibody-based biosensors 2967.13 Therapeutic antibodies 2977.14 The future uses of antibody technology 2997.15 Suggestions for further reading 2998 Protein structure, purification, characterisationand function analysis300J. WALKER8.1 Ionic properties of amino acids and proteins 3008.2 Protein structure 3048.3 Protein purification 3078.4 Protein structure determination 3288.5 Proteomics and protein function 3408.6 Suggestions for further reading 3519 Mass spectrometric techniques352A. AITKEN9.1 Introduction 3529.2 Ionisation 3549.3 Mass analysers 3599.4 Detectors 3779.5 Structural information by tandem mass spectrometry 3799.6 Analysing protein complexes 3909.7 Computing and database analysis 3949.8 Suggestions for further reading 39710 Electrophoretic techniques399J. WALKER10.1 General principles 39910.2 Support media 40310.3 Electrophoresis of proteins 40710.4 Electrophoresis of nucleic acids 42210.5 Capillary electrophoresis 42710.6 Microchip electrophoresis 43110.7 Suggestions for further reading 432vii Contents